PCR Protocols in Molecular Toxicology, 1st Edition – FULL INSTANT DOWNLOAD | PDF DOCX EPUB

Name: PCR Protocols in Molecular Toxicology
Edition: 1st Edition
ISBN-13: 9781138473690
ISBN-10: 1138473693
Addition ISBN: 9780849333446, 9781439805800, 9780429186936, 9780429540790, 0429526091, 9780429526091
Author(s): John P. Vanden Heuvel, Publisher: CRC Press, Copyright: 1997
Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression Differential display cloning Cloning and library screening by PCR Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes Basic, non-PCR based molecular biology methods PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.